The Distribution of Immunoglobulin-containing Cells in the Goat Mammary Gland: A Morphometric and Immunohistochemical Study.

Hiader Ibrahim Ismail, Yasohiru Kon

Abstract


The distribution of immunoglobulin (Ig)-containing cells in the mammary gland of the goat was studied at different stages of the reproductive cycle using immunohistochemical techniques. Generally, Ig-containing plasma cells (IgA, IgG and IgM) in the mammary gland were abundant during pregnancy, increase in number after parturition and gradually decrease in number, especially in the post weaning period. IgA-containing cells were the predominant cell type among the plasma cells throughout the reproductive cycle. Their number increased at late pregnancy, being maximum at the colostrum period and decreased thereafter. IgG- and IgM-containing plasma cells were fewer at all stages of the reproductive cycle; the former were slightly more than the latter. Both types of cells followed a distribution pattern similar to that shown by IgA-containing cells. The rich existence of plasma cells at late pregnancy and colostrum period suggests that their main role is transfer of passive immunity to the neonates rather protection of the mammary gland.


Keywords


Immunoglobulin-containing cells, Plasma cells, Immunohistochemistry, goat.

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References


Almogren, A., Senior, W.B., and Kerr, M.A. (2007). A comparison of the binding of secretory component to immunoglobulin A (IgA) in human colostral S-IgA1 and S-IgA2. Immunology, 120, 273-280.

Bsitany, T.S., and Tomasi, T.B. (1970). Serum and secretory immunoglobulin’s of the rat. Immunohistochemistry, 7, 453-460.

Brandtzaeg, P. (1983). The secretory immune system of lactating human mammary glands compared with other exocrine organs. Annals of the New York Academy of Science, 409, 353-382.

Brown, P.J., Bourne, F.J., and Denny, H.R. (1975). Immunoglobulin-containing cells in pig mammary gland. Journal of Anatomy, 120, 329-335.

Butler, J.E., Maxwell, C.F., Pierce, C.F., Hylton, M.B., Asofsky, R., and Kiddy, C.A. (1972). Studies on the relative synthesis and distribution of IgA and IgG in various tissues and body fluids of the cow. Journal of Immunology, 109, 38-46.

Chernishov, V.P., and Slukvin, I.I. (1990). Mucosal immunity of the mammary gland and immunology of mother/newborn interrelation. Arch Immunol Ther Exp, 38, 145-64.

Eddie, D.S., Schulkind, M.L., and Robbins, J.B. (1971). The isolation and biologic activities of purified secretory IgA and IgG anti-Salmonella typhimurium “O” antibodies from rabbit intestinal fluid and colostrum. Journal of Immunology, 106,181-190.

Guidry, A.J., Butler, J.E., Pearson, R.E., and Weinland, B.T. (1980). IgA, IgG1, IgG2, IgM, and BSA in serum and mammary secretion throughout lactation. Veterinary Immunology and Immunopathology, 1, 329-341.

Hidiriglou, M., Batra, R.A., and Nielsen, K.H. (1992). Effect of vitamin E supplementation and of health status of mammary gland on immunoglobulin concentration in milk of dairy cows. Annales de Recherches Véterinaires, 23, 139-144.

Hurley, W.L., and Theil, P.K. (2011). Perspectives on Immunoglobulins in Colostrum and Milk. Nutrients, 3, 442-474.

Ismail, H.I., and Kon, Y. (2015). Immunological quantitation of IgG and IgM in milk and serum of the goat at different stages of the reproductive cycle. Annals of Biological Research, 6, 16-20.

Ismail, H.I., Hashimoto, Y., Kon, Y., Davis, W.C., and Iwanga, T. (1996). Lymphocyte subpopulations in the mammary gland of the goat. Veterinary Immunology and Immunolpathology, 25, 201-212.

Larson, B.L., Heary, H.L., and Devery, J.E. (1980). Immunoglobulin production and transport by the mammary gland. Journal of Dairy Science, 63, 655-671.

Lee, C.S., and Lascelles, A.J. (1970). Antibody-producing cells in antigenically stimulated mammary glands and in the gastro-intestinal tract of sheep. Australian Journal of Experimental Biology and Medical Sciences, 48, 525-535.

Lee, C.G., Ladds, P.W., and Watson, D.L. (1978). Immunocyte populations in the mammary gland of the rat at different stages of pregnancy and lactation. Research in Veterinary Science, 24, 322-327.

Marx, G.D., and Carulo, E.V. (1963). Method of biopsy of the mammary gland of cows and goats. Journal of Dairy Science, 46, 576-579.

McLean, I.W., and Nakane, P.K. (1974). Periodate-lysine-paraformaldehyde fixative for immunoelectron microscopy. Journal of Hisyochemistry and Cytochemistry, 22, 1077-1083.

Nickerson, S.C. (1985). Immune mechanisms of the bovine udder: An overview. Journal of the American Veterinary Medical Association, 187, 41-45.

Norcross, N.L. (1991). Specific defense mechanisms of the udder. Flemish Veterinary Journal, 62, 129-139.

Penhale, W.J. and Christie, G. (1969). Quantitative studies on bovine immunoglobulins:Adult plasma and colostrum levels. Research in Veterinary Science, 10, 493-501.

Sordillo, L.M., and Nickerson, S.C. (1988). Quantification and immunoglobulin classification of plasama cells in the nonlactating bovine mammary tissue. Journal of Dairy Science, 71, 84-91.

Sordillo, L.M., Oliver, S.P., Guidry, A.J., and Dermody, J.T. (1988). Humoral immune response of bovine mammary glands colonized with Corynebacterium bovis: Enumeration of plasma cell populations in tissue and immunoglobulin concentration in milk. Journal of Veterinary Medicine B., 35, 617-627.

Weibel, E.R. (1969). Steriological principles for morphometry in electron microscopic cytology. International Review of Cytology, 26, 235-302.

Weisz-Carington, P., Roux, E.M., and Lamm, E.M. (1977). Plasma cells and epithelial immunoglobulin’s in the mouse mammary gland during pregnancy and Lactation. Journal of Immunology, 119, 1306-1309.

Yurchak, A.M., Butler, J.A., and Thmasi, T.B. Jr. (1971). Fluorescent localization of immunoglobulin’s in the tissues of the cow. Journal of Dairy Science, 54, 1324-1325.

Van der Feltz, M.J., de Groot, N., Bayley, J.P., Lee, S.H., Verbeet, M.P., and de Boer, H.A. (2011). Lymphocyte homing and Ig secretion in the murine mammary gland. Scand J. Immunol, 54, 292-300.


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